BRAP (BRAP2, BRCA1 Associated Protein, EC 6.3.2., Galectin 2 Binding Protein, IMP, Impedes Mitogenic Signal Propagation, Renal Carcinoma Antigen NY REN 63, RING finger Protein 52, RNF52, Zgc:92894)

Antibodies / Antibodies-Transcription Factors, Zinc (Ring) Finger

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to human BRAP protein fragments expressed in E. coli.

Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.