PLCB4 (Phospholipase C Beta 4, PI-PLC, Phospholipase C-beta-4, Phosphoinositide Phospholipase C-beta-4, 1-phosphatidylinositol 4,5-bisphosphate Phosphodiesterase beta-4)

Antibodies / Antibodies-Enzymes, Phosphodiesterase

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

PLCb4 (Ala2ca.Gln250)

In the phosphoinositide (PI) cycle, phospholipase C (PLC) catalyzes hydrolysis of a plasma membrane phospholipid, phosphatidylinositol 4, 5-bisphosphate, generating 2 second messengers, the water soluble 1, 4, 5-inositol trisphosphate and the membrane-associated 1, 2-diacylglycerol. In mammalian tissues, several groups of PLCs have been characterized, including PLC-beta, and each group contains at least 3 isoforms. PLCb4 catalyzes the formation of inositol 1, 4, 5-trisphosphate and diacylglycerol from phosphatidylinositol 4, 5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals in the retina. Two transcript variants encoding different isoforms have been found for this gene.

Applications: 
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Immunocytochemistry: 1:50-500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.