Acetylcholinesterase YT blood group (Acetylcholine acetylhydrolase, ACHE, precursor, ARACHE, N-ACHE) (Biotin)

Antibodies / Antibodies-Enzymes, Acetylcholinesterase

-20°C

Supplied as a lyophlized poweder from PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Labeled with Biotin.

Acetyl Cholinesterase (Bovine Erythrocytes)

Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.

Applications:
Suitable for Immunoblotting (Western or Dot Blot), ELISA, Immunomicroscopy and Immunohistochemistry as well as other antibody based assays using streptavidin or avidin conjugates. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000-1:2000 1ug Acetyl Cholinesterase of was used in a standard capture ELISA with Streptavidin (HRP) S7973-97A and ABTS (2, 2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) A0030 as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.

Label:
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)

Biotin/Protein Ratio:
10-20 BAC molecules per Goat IgG molecule

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.