Angiotensin Converting Enzyme 2 (ACE-2, ACEH)

Antibodies / Antibodies-ACE

-20°C

Supplied as a liquid in PBS, pH 7.5, 0.1% BSA.

Synthetic peptide corresponding to 20aa of mouse ACE-2 (KLH). Cellular Localization: Extracellular. Species Sequence Homology: rat; 90%, human, chimp, feline; 65%.

Renin-Angiotensin System (RAS) is a crtical regulator of blood pressure homeostasis. The protease renin cleaves angiotensinogen into inactive decameric peptide angiotensin-I (Ang-I). Angiotensin-converting enzyme (ACE) then cleaves C-terminal dipeptide from Ang-I to form an active octamer angiotensin-II (Ang-II), which can contribute to hypertension by promoting vascular smooth muscle vasocontriction and renal tubule sodium reabsorption. ACE can also cleave many other small peptides including the vasodialating peptide bradykinin into inactive fragment, cleave Alzheimer amyloid beta-peptide (Abeta), retard Abeta aggregation, deposition and fibril formation. ACE mutant mice display spontaneous hypotension, partial male infertility and kidney malformations. ACE is found in somatic (s-ACE) and testicular/germinal (t-ACE) isoforms. The products of renin and ACE catalysis, namely Ang1-10 and Ang1-8 can also be by another peptidase, ACE-2 to Ang1-9 and Ang1-7, respectively. ACE-2 and ACE (s-ACE and t-ACE) are made as transmembrane (TM) proteins. These enzymes also exist as soluble, truncated forms lacking the TM and cytosolic domains.

Applications:
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.

Recommended Dilutions:
Western Blot (Reducing and Non-reducing): 1-10ug/ml (ECL)
ELISA: 0.5-1ug/ml. Coat ELISA plates at 1ug/ml with Control Peptide.
Optimal dilutions to be determined by the researcher.

Control Peptide:
A2295-01J: Angiotensin Converting Enzyme 2, Mouse (ACE-2, ACEH)

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.