Angiotensin Converting Enzyme 2, IN2 (ACE-2, ACEH)

Antibodies / Antibodies-ACE

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

Synthetic peptide corresponding to amino acids 169 to 183 of human ACE2

Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology (1-2). In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus (3). The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function (4).

Applications:
Can be used for the detection of ACE2 by Western blot at 0.5 to 2ug/ml. Human kidney or human testis cell lysate can be used as positive control and a 90 kD band can be detected.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.