Angiotensin Converting Enzyme 2, Ectodomain (ACE2, ACE-2, ACE-related Carboxypeptidase, Angiotensin Converting Enzyme Homolog, ACEH, DKFZP434A014, EC 3.4.17, OTTHUMP00000022963)

Antibodies / Antibodies-ACE

-20°C

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul PBS.

NS0-derived recombinant human ACE-2 ectodomain aa18-740; Q9BYF1.

Angiogensin I Converting Enzyme-2 (ACE-2) is a type I transmembrane zinc protease that cleaves angiotensins I and II to produce vasodilatory and anti-proliferative peptides. The balance between ACE-1 and ACE-2 activity is critical for maintaining cardiovascular, renal, and pulmonary function. ACE-2 isoforms of 75kD and 120kD are differentially expressed between lung and kidney, respectively, and a shed soluble form is generated by TACE/ADAM17 mediated cleavage. ACE-2 also functions as the cellular uptake receptor for the SARS coronoavirus. Within the extracellular domain, human ACE-2 shares 83% aa sequence identity with mouse and rat ACE-2.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 2ug/ml NS0 mouse myeloma cell line and human kidney tissue
Immunoprecipitation: 25ug/ml conditioned cell culture medium spiked with recomninant human ACE-2
Immunohistochemistry: 1-25ug/ml on frozen sections of rat kidney and paraffin-embedded sections of hamster lung.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer..