Bcl 6 (B Cell Leukemia 6, Lymphoma 6, Zinc finger protein 51 LAZ3)

Antibodies / Antibodies-Apoptosis

-20°C

Supplied as a liquid in 0.09% sodium azide.

Recombinant protein corresponding to aa 3-484 of the human Bcl-6 protein. Cellular Localization: Nuclear. Epitope: aa3-484.

Bcl 6 is mainly expressed in normal germinal center B cells and related lymphomas. Bcl 6 is involved in chromosome rearrangements at 3q27 in non-Hodgkin’s lymphomas and Bcl 6 rearrangements have been detected in 33%-45% of diffuse large B cell lymphomas. Bcl 6 has been detected immunohistochemically in follicular lymphomas, diffuse large B cell lymphomas, Burkitt’s lymphomas and in nodular, lymphocyte predominant Hodgkin’s disease

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Formalin/Paraffin): 1:10 for 60 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0 for 10-20 minutes, followed by cooling at RT for 20 minutes. Requires use of a high sensitivity detection system.
Optimal dilutions to be determined by the researcher.

Positive Control:
Tonsil

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.