Bcl X (Bcl-X, BclX, Apoptosis Regulator Bcl X, Apoptosis Regulator Bcl-X, B cell Lymphoma 2-like, Bcl-2-like 1 Protein, Bcl xL, Bcl-xL, Bcl xL/S, Bcl-xL/S, Bcl xS, Bcl-Xs, Bcl2 Like 1, Bcl2-like 1, Bcl2L1, Bcl2L, DKFZp78

Antibodies / Antibodies-Apoptosis

-20°C

Supplied as a liquid in Tris-citrate/phosphate, pH 7-8, 0.09% sodium azide.

Synthetic peptide made to an internal portion of the human protein (within residues 1-50). Mitochondrion membrane; Single-pass membrane protein (By similarity). Nucleus membrane; Single-pass membrane protein; Cytoplasmic side (By similarity).

Bcl-x, a member of the Bcl-2 protein family, inhibits cell death, or apoptosis (1). Bcl-x is expressed as two isomeric forms, Bcl-xL and Bcl-xS, and is typically present in the cytosol in association with the mitochondrial membrane. Bcl-xL forms heterodimers with various proteins, including Bax, Bak and Bcl-2 (2). It has been found that heterodimerization with Bax does not seem to be required for anti-apoptotic activity (3). Since Bcl-xL can form an ion channel in synthetic lipid membranes, there is a strong possibility that this property plays a role in heterodimerization-independent cell survival (4). The Bcl-X(S) isoform promotes apoptosis.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot 1:10000, a band is seen ~26 kD
Optimal dilution determined by the researcher.

Positive Controls:
A431 Whole Cell Lysate

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.