Brain-Specific Angiogenesis Inhibitor 3 (BAI3)

Antibodies / Antibodies-Neuroscience

-20°C

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

NS0-derived rhBAI3. (aa 25 - 880)

Human BAI3 (brain-specific angiogenesis inhibitor 3) is a 177kD, 7-transmembrane (TM) member of the secretin receptor family. It is synthesized by neurons of the CNS and likely is a negative regulator of angiogenesis. BAI3 is 1498 amino acids (aa) in size. It contains three distinct regions; an N-terminal extracellular domain (ECD) (aa 25-883), a 7-TM segment, and a C-terminal cytoplasmic region. The ECD contains four antiangiogenic TSP type1 repeats (aa 296-508), and one GPS domain (aa 816-867) that is likely used to cleave the ECD from the membrane-bound receptor. There is one alternate splice form that shows a deletion of aa 643-665. Over aa 25-880, human BAI3 is 98% aa identical to mouse BAI3.

Applications:
Suitable for use in Flow Cytometry, Direct ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 8-25ug/ml in Immersion fixed paraffin-embedded sections of human brain (cortex)
Flow Cytometry: 0.25ug/10e6 cells in HEK293 Human Cell Line Transfected with Human BAI3 and eGFP
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.