BTF3 (Basic Transcription Factor 3, Nascent Polypeptide-associated complex, β/NACB)

Antibodies / Antibodies-Transcription Factors

-20°C

Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute in PBS containing 0.02% sodium azide.

Purified, E. coli-derived recombinant human BTF3 rhBTF3; aa 2-162; Accession# P20290).

BTF3 (basic transcription factor 3; also nascent polypeptide-associated complex β/NACB) is a member of the NAC-beta family of transcription factors. It is ubiquitously expressed and involved in the initiation of gene transcription by RNA polymerase. BTF3 also appears to impact estrogen receptor alpha (ERα) stimulation of gene activation by binding to both ligand activated and non-ligand activated ERα. Human BTF3 is 206 amino acids (aa) in length and contains one potential phosphorylation site at Ser30, and a NACB domain (aa 82-147). There are multiple potential splice variants. Two variants show alternate start sites, one at Met45 (called BTF3b), and another at Met108.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.