CBLB, ID (E3 Ubiquitin-protein Ligase CBL-B, Signal Transduction Protein CBL-B, SH3-binding Protein CBL-B, Casitas B-lineage Lymphoma Proto-oncogene b, RING Finger Protein 56, Nbla00127, RNF56) (APC)

Antibodies / Antibodies-Transcription Factors, Zinc (Ring) Finger

4°C Do Not Freeze

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 103-130 in the central region of human CBLB, UniProt Accession #Q13191.Species Sequence Homology: Xenopus, mouse and rat

E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. It also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. It may also be involved in EGFR ubiquitination and internalization.

Applications:
Suitable for use in FLISA, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Formalin-fixed, paraffin-embedded sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.