c-fos Oncoprotein (Fos)

Antibodies / Antibodies-Oncoproteins

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

Synthetic peptide: Met-Phe-Ser-Gly-Phe-Asn-Ala-Asp-Tyr-Glu-Ala- Ser-Ser-Ser-Arg-Cys, selected from a conserved region of mouse and human c-fos.

c-fos is a 55kD nuclear associated protein that is rapidly induced in HL60, HeLa and NIH3T3 cells by a variety of agents. c-fos is also induced in neuronal tissue following drug induced seizures.

Applications:
Suiatble for use in Western Blot and Immunocytochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: Detergent lysates were produced from HeLa and HL-60 cells stimulated to express c-fos protein. Detects a band at 55-60kD on immunoblots of stimulated HeLa and HL-60 lysates.
Immunocytochemistry: Has been used in immunocytochemistry of vibratome sections of rat brains following either surgical damage to one of the hemispheres (detects c-fos and c-fos related proteins in the nuclei of neurons in the damaged hemisphere) or kainic acid treatment.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.