c-Fos, phosphorylated (Ser374) (Fos)

Antibodies / Antibodies-Oncoproteins

-20°C

Supplied as a lyophilized powder from 2X PBS, PEG, and sucrose, 0.09% sodium azide. Reconstitute with 1ml of water for 15 min. at RT.

Synthetic peptide derived from human c-Fos sequence phosphorylated at Ser374 (conjugated to KLH)

The immediate early gene product c-Fos is expressed following mitogenic stimulation and functions as a sensor for MAPK signal duration. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at Ser374. Phosphorylation stabilizes the c-Fos protein and primes c-Fos for additional phosphorylation at Thr325. During transient MAPK activation, activity declines before accumulation of the c-Fos protein.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 0.1ug/ml
Immunohistochemistry: 1:50
Western Blot: 0.5ug/ml (ECL).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.