Calretinin (Calbindin, CAB29, CAL2)

Antibodies / Antibodies-Calretinin

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Recombinant rat calretinin

Calretinin is a neuron specific calcium binding protein (31kD) found mostly in the CNS and retina. It is present in subsets of neurons throughout the brain and spinal cord, including sensory ganglia. It shares about 55% amino acid homology with calbindin, another calcium binding protein found in brain and periphery. Studies suggest that calretinin, like calbindin, may be neuroprotective.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western blot: 1:1, 000-1:3, 000
Immunohistochemistry (on rat brain tissue): IFA: 1:1, 000-1:5, 000
PAP: 1:1, 000-1:5, 000
The antibody has been used successfully on formalin-fixed, paraffin embedded human tissue after pre-treatment of the tissue slides with citrate buffer and heat treatment. Suggested starting dilution 1:100-1:400. Proteolytic digestion with pepsin is detrimental to the immunostaining.
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.