Carboxypeptidase Y

Antibodies / Antibodies-Enzymes, Carboxypeptidase

-20°C

Supplied as a liquid from PBS, pH 7.2, 0.01% sodium azide

Carboxypeptidase Y (yeast).

Applications:
Suitable for use in Immunoblot (Western or Dot Blot), ELISA, Immunoprecipitation and Conjugation. Other applications have not been tested.

Recommended Dilution:
ELISA: 1:10, 000-1:42, 000
Western Blot: 1:2500. Both the antiserum and IgG fractions of anti-carboxypeptidase Y (Baker’s yeast) are shown to detect under reducing conditions of SDS-PAGE the 61kD enzyme in cellular extracts. Approximately 10ug of total protein is loaded per lane. A 1:5, 000 dilution of the primary antibody is used followed by detection using HRP goat-a-rabbit IgG diluted 1:4, 000 and color development using 4-CN substrate until sufficient color develops.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.