CARD8 (Caspase Recruitment Domain, Cardinal/TUCAN)

Antibodies / Antibodies-Apoptosis, Caspase

-20°C

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

Partial recombinant sequence from human CARD8

Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARDcontaining protein was recently identified and designated CARD8. This protein interacts with DRAL, a p53-responsive protein implicated in the induction of apoptosis, and caspase-1 and its related proteins ICEBERG and pesudo- ICE. Although there are conflicting reports on whether CARD8 acts a pro- or anti-apoptotic protein, it has been suggested that it functions as an adaptor molecule regulating caspase-1 and NF-£eB activation.

Applications:
Suitable for use in Immunofluorescence/Immunocytochemistry, Flow Cytometry, Western Blot, Dot Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 0.5-2ug/ml
Western Blot: 0.2-2ug/ml
Immunofluorescence (IC): 2-100ug/ml
Immunoprecipitation: 100-500ug/sample
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.