CD3

Mab

Bovine, Canine, Equine, Feline, Chicken, Human, Monkey, Mouse, Porcine, Rabbit, Xenopus

-20°C

Supplied as a liquid in PBS, 0.09% sodium azide.

Synthetic peptide corresponding to sequence, ERPPPVPNPDYEPC, derived from cytoplasmic epitope of human CD3.
Species Sequence Homology: mammals, birds, amphibia

The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intracytoplasmic; however, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells and is present in majority of T cell neoplasms. CD3 antigen is expressed on approximately 60–80% of human peripheral blood lymphocytes, 20–40% of splenic lymphocytes, the majority of T-CLL and approximately 70% pf T-All.
The CD3 complex consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kD.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:50-1:100 Requires membrane permeabilization
Immunohistochemistry (Frozen): 1:100
Immunohistochemistry (Paraffin): 1:100. Requires antigen retrieval using heat treatment using Tris/EDTA buffer pH 9.0 prior to staining of paraffin sections.
Optimal dilutions to be determined by the researcher.

Positive for Immunohistochemistry:
Tonsil

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.