CD104 (CD104 Antigen, Integrin beta-4, Integrin beta4, ITGB4, AA407042, C230078O20)

Mab

IgG2a, k

4°C (-20°C Glycerol)

Supplied as a liquid in 100mM borate buffered saline, pH 8.0, before the addition of glycerol to 40%. Preservative free.

Tumor-associated antigen TSP-180 immunoaffinity purified from a transplantable BALB/c mouse lung cell carcinoma

CD104 represents the integrin beta4 subunit that associates non-covalently with integrin alpha6 (CD49f) to form the alpha6beta4 integrin heterodimer.2, 3 The alpha6beta4 complex is expressed on the basal surface of a variety of epithelial cell types, particularly on stratified squamous epithelia. It is also found in peripheral nerves, in certain subsets of endothelial cells, and on immature thymocytes, and has been found to be associated with a number of malignant tissues.3-5, 6 Hemidesmosomal alpha6beta4 plays an important role in the adhesion of epithelial cells to basement membranes via interactions with laminin and/or kalinin anchoring filaments.7 It is not known if the antibody can block this adhesion.

Applications:
Suitable for use in Immunohistochemistry, Immunoprecipitation
and Western blot. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: 1-5ug/ml (frozen sections)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.