CD146 (Mucin 18, MUC18 Glycoprotein, Melanoma Associated Antigen A32, S-endo 1 Endothelial Associated Antigen, Melanoma Adhesion Molecule Mel-CM) (Biotin)

Antibodies / Antibodies-CD Markers

-20°C

Supplied as a liquid in PBS, pH 7.6, 15mg/ml BSA, 0.09% sodium azide. Labeled with Biotin.

HUVEC

This antibody does not stain carcinoma cell lines HT-29, COLO205 or M21; the T cell lines Jurkat and HuT78; fibroblasts; HL-60 or CHO cells; or EBV transformed B cell lines, although it is expected that CD146 expression is present in many tumor lines. It does not stain normal monocytes, granulocytes, red cells, platelets, T cells or B cells from marrow or peripheral blood; nor does it detect marrow megakaryocytes or the megakaryoblast line HU3. The peripheral blood cells that do stain are positive for both von Willebrand Factor (vWF) and thrombomodulin (the combined expression of which is limited to endothelium), and they stain for flt and flk. In recent testing, melanoma A2058, SKMEL.3 and A375.S2 are positive, while murine melanoma M3 is negative. One smooth muscle line (HISM) was positive and one negative (TIGHA-VSMC). Human blood lymphoctes are negative with or without prior stimulation. This biotinylated format is particularly useful for studies on mouse tissues when combined with streptavidin-FITC.

Applications:
Suitable for use in Flow Cytometry, ELISA, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1-10ug/ml
ELISA: 1-10ug/ml
Immunoprecipitation: 1-10ug/ml
Immunohistochemistry (Light paraformaldehyde fixation): 1-10ug/ml
Immunocytochemistry: 1-10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.