CD273 (CD273 Antigen,B7-DC,bA574F11.2,Btdc,Butyrophilin B7-DC,Butyrophilin B7DC,MGC142238,MGC142240,Programmed Cell Death 1 Ligand 2,PD-1 Ligand 2,PDCD1 Ligand 2,PDCD1L2,PDCD1LG2,Programmed Death Ligand 2,PDL2,PD-L2) (FI

Antibodies / Antibodies-CD Markers

-20°C

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

B7-DC transfected RAW264.7 cells.

CD273 is a 42kD member of the B7 family, otherwise known as B7-dendritic cell (B7-DC), or programmed death ligand-2 (PD-L2). The expression of CD273 is restricted to a subpopulation of dendritic cells (DCs), and DCs and macrophages that have been stimulated with interferon gamma, GM-CSF or IL-4. CD273 has been identified as a ligand for PD-1(CD279), along with another B7 family member B7-H1 (CD279). Studies suggest that both CD273 and B7-H1 co-stimulate or inhibit T cell proliferation and cytokine production.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat-1:5; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.