Collagen Type I

Antibodies / Antibodies-Collagen

-20°C

Supplied as a liquid in 100mM borate buffered saline, pH 8.2. No preservatives added.

Human placental collagen type I isolated by limited pepsin digestion and selective salt precipitation.

Type I collagen is the most common of the collagens in vertebrates. It comprises up to 90% of the skeletons of the mammals and is also widespread all over the body. Such as: skin, tendons, ligaments, cornea, intervertebral disks, dentine, arteries and granulation tissues as the main locations. The importance of type I collagen for medical research is that it is involved in many human and animal diseases, including fibrosis, osteoporosis, cancer, atherosclerosis etc. Type I
collagen is frequently utilized to monitor physiological changes in tissues as well as being used as diagnostic tools in various pathological conditions.

Applications:
Suitable for use in Dot and Slot Blot, ELISA, Indirect Immunohistochemistry and Indirect Immunocytochemistry. Not recommended for paraffin tissue sections. Other applications not tested.

Recommended Dilutions:
Dot and Slot Blot: 1:100-1:500
ELISA: 1:50-1:100
Immunohistochemistry (Indirect, frozen sections only): 1:10-1:20
Immunocytochemistry (Indirect): 1:10-1:20
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.