Complement C3, C3b

Antibodies / Antibodies-Complement

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.02% sodium azide.

Native human C3

The complement system is an important factor in innate immunity. The third complement component, C3, is central to the classical, alternative and lectin pathways of complement activation. The synthesis of C3 is tissue-specific and is modulated in response to a variety of stimulatory agents. C3 is the most abundant protein of the complement system with serum protein levels of about 1.3mg/ml. An inherited deficiency of C3 predisposes a person to frequent bacterial infections. C3 fragments are deposited in tissues at sites of antibody-mediated immunopathology. In ulcerative colitis and idiopathic chronic inflammatory bowel disease, the deposition of C3 in the diseased mucosa has been reported. After activation of the complement system, certain enzymes become active, resulting in the cleavage of C3 into C3b and the anaphylatoxin C3a. C3b becomes attached to immune complexes and is further cleaved into iC3b, C3c, C3dg and C3f. Within the alternative pathway of complement, C3b plays a critical role in the amplification loop initiated by spontaneous hydrolysis of C3.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:50 detects a band at ~100kD (reduced) and at ~190kD (non-reduced)
Immunohistochemistry (paraffin): 1:200
Immunohistology (Frozen): 1:50.
Optimal dilutions to be determined by the researcher.

Positive Control:
Human serum

Negative Control:
C3 deficient serum

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.