Csk (C-Terminal Src Kinase, C-Src Kinase, c-Src Tyrosine Kinase, Tyrosine Protein Kinase CSK, Protein-Tyrosine Kinase CYL)

Antibodies / Antibodies-Protein Kinases

-20°C

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

Bacterially expressed recombinant fragment of human Csk corresponding to aa330-450.

C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase. One of its major functions is to specifically phosphorylate a conserved C-terminal tyrosine of Src family kinases thereby down-regulating their kinase activity. CSK is expressed ubiquitously as a protein of approximately 50kD in cytosol, but a fraction distributes to lipid rafts due to interaction with the lipid raft-associated protein PAG (Csk-binding protein).

Applications:
Suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:250-1:300. Weak non-specific band of ~60kD is detected in human cell lines at lower antibody dilutions.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.