Cyclin D1, NT (CCND1 Protein, Cyclin D1, Isoform CRA_c, cDNA, FLJ93625, Homo Sapiens Cyclin D1 (PRAD1: Parathyroid Adenomatosis 1) (CCND1),mRNA, Cyclin D1, PRAD1: Parathyroid Adenomatosis 1, CCND1)

Antibodies / Antibodies-Cyclin

-20°C

Supplied as a liquid in PBS, 0.09% sodium azide.

Synthetic peptide selected from the N-terminal region of human CCND1 (KLH).

CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of the gene encoding this protein, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1, 000
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.