Desmin (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, intermediate filament protein)

Antibodies / Antibodies-Extracellular Matrix Proteins

-20°C

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

Synthetic peptide corresponding to the C-terminus of RDGEVVSEATQQQHE. Species sequence homology: canine, porcine and bovine

Desmin is an intermediate filament protein of both smooth and striated muscles. Antibody to desmin reacts with striated (skeletal and cardiac) as well as smooth muscle cells. In skeletal and cardiac muscles, the staining is confined to the Z-bands giving a characteristic striated appearance. Anti-desmin antibody is useful in identification of tumors of myogenic origin. It reacts with leiomysarcomas (smooth muscle) as well as rhabdomysarcomas (striated muscle).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Peptide ELISA: 1:4000
Western Blot: 0.1-0.3ug/ml ~55-60kD band observed in human, mouse, rat and porcine Skeletal Muscle lysates (calculated MW of 53.5kD according to NP_001918.3).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.