EphB2 (EPH Receptor B2, CAPB, DRT, Ephrin type-B Receptor 2, EPHT3, EPTH3, ERK, Hek5, HEK5, PCBC, Tyro5, TYRO5, Receptor Protein-tyrosine Kinase HEK5, Tyrosine-protein Kinase Receptor EPH-3, Renal Carcinoma Antigen NY-REN-47, Tyrosine-protein Ki

Antibodies / Antibodies-Tyrosine Kinases

-20°C

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

NS0-derived, recombinant mouse EphB2 extracellular domain aa27-551; P54763.

EphB2, also known as Cek5, Nuk, Erk, Qek5, Tyro5, Sek3, Hek5, and Drt, is a 125kD member of the transmembrane Eph receptor tyrosine kinase family. The 90kD extracellular domain (ECD) can be shed from neurons by calcium ionophores or NMDA receptor agonists. EphB2 interaction with Ephrin-A5 promotes axonal growth cone collapse, while its interaction with Ephrin-B ligands is required for inner ear, renal, urorectal, and vascular development. Within the ECD, mouse EphB2 shares 99% aa sequence identity with human and rat EphB2.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 2ug/ml using lysates of mouse brain tissue
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.