ERK1,NT (Extracellular Signal-regulated Kinase 1,ERK-1,ERT2,Insulin-stimulated MAP2 Kinase,MAP Kinase Isoform p44,Microtubule-associated Protein 2 Kinase,Mitogen-activated Protein Kinase 3,MAP Kinase 3,MAPK 3,MAPK3,Mitog

Antibodies / Antibodies-Protein Kinases

-20°C

Supplied as a liquid in PBS, 0.09% sodium azide.

Synthetic peptide selected from the N-terminal region of human ERK1 (KLH).

ERK1, a member of the MAP kinase subfamily of Ser/Thr protein kinases, is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. It phosphorylates EIF4EBP1 and is required for initiation of translation. The protein also phosphorylates microtubule-associated protein-2 (MAP2). ERK1 is activated and tyrosine phosphorylated in response to insulin and NGF.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1, 000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.