FAK (Focal Adhesion Kinase 1, FADK 1, pp125FAK, Protein-tyrosine Kinase 2, PTK2, FAK1)

Antibodies / Antibodies-Tyrosine Kinases

-20°C

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

Synthetic non-phosphopeptide corresponding to human FAK around the phosphorylation site of tyrosine 397 (D-D-YP-A-E).

Focal Adhesion Kinase (FAK) is a 125kD non-receptor protein tyrosine kinase that is a substrate for Src and a key element in growth factor and integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Tyrosine 861 of FAK is a major Src phosphorylation site that promotes the binding of FAK to the cytoplasmic tail integrins and is also involved in cancer.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1, 000
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.