Fos, phosphorylated (Thr232) (c-Fos)

Antibodies / Antibodies-Proteins

-20°C

Supplied as a liquid in PBS (without Mg2+ and Ca2+) pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.

Synthetic phosphopeptide derived from the region of human c-Fos that contains threonine 232. The sequence is conserved in mouse and rat.

c-Fos is a ~60kD immediate early gene product and a member of AP-1 family. c-Fos dimerizes with c-Jun to form the transcriptionally active complex AP-1, and functions in regulating cell proliferation and differentiation. c-Fos expression and activation are induced in many tumors including breast cancer), and in response to a variety of stimuli (including growth factors, cytokines and stress). ERK1&2 mediate growth factor-induced c-Fos induction and transcripional activation by directly phosphorylating the carboxyl terminal domain of c-Fos. c-Fos is phosphorylated by Ras on threonine 232, the homologue of serine 73 of c-Jun, which results in augmenting its transcriptional activity.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.