GCET2 (Germinal Center B Cell-expressed Transcript 2 Protein, Germinal Center-associated Lymphoma Protein, hGAL, GAL) (HRP)

Antibodies / Antibodies-Cancer Markers

-20°C

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

Full length recombinant protein corresponding to aa1-179 from human GCET2 with GST tag (AAH30506). MW of the GST tag alone is 26kD.

GCET2, commonly known as germinal center B cell expressed transcript 2 protein, is a 178aa protein expressed in diffuse large B cell lymphoma (DLBCL) and several germinal center (GC) like lymphoma cell lines (at protein level). It is highly expressed in normal GC lymphocytes and GC derived malignancies, as well as being present in the thymus and spleen. In B cells expression of the HGAL gene is specifically induced by interleukin 4.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1-5ug/ml using recombinant protein.
Western Blot (Cell lysate): 1-5ug/ml. Specific band identified at ~21kD using Jurkat lysate.
Western Blot (Recombinant lysate protein): 1-5ug/ml. Specific band of ~45kD identified using immuogen protein lysate.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.