GFAP (Glial Fibrillary Acidic Protein)

Antibodies / Antibodies-Growth Factors, Cytokines

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.

GFAP (Glial Fibrillary Acidic Protein) from human brain

Immunohistochemical studies have shown that GFAP is selectively located in astrocytes and represents the major constituent of astrocytic intermediate filaments. GFAP expression levels are highly variable during development of the central nervous system. As differentiation proceeds, neurons are generated at the ventricular surface and then migrate along astrocytic processes toward their ultimate destination, whereas astrocytes provide the scaffolding and throphic support required for neuronal positioning. Astrocytes and oligodendrocytes together make up half the cell content in human brain. In adults, GFAP levels increase as a result of the proliferation of astrocytes that occurs in a response to a variety of physical, chemical and etiological insults, including Alzheimer’s disease, epilepsy and multiple sclerosis.

In the peripheral nervous system GFAP is expressed by Schwann cells. Upon differentiation, myelin forming Schwann cells down-regulate GFAP, whereas in non-myelin forming Schwann cells GFAP persists into adulthood.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (ABC): 1:25-1:200. Can be used with frozen and paraffin sections
Western Blot: 1:100-1:1000
Optimum dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.