Glial Fibrillary Acidic Protein (GFAP, FLJ45472) (PE)

Antibodies / Antibodies-Growth Factors, Cytokines

4°C Do Not Freeze

Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).

Bovine spinal cord homogenate.

Glial fibrillary acidic protein, or GFAP, is a class III intermediate filament. During development of the central nervous system, GFAP distinguishes astrocytes from other glial cells. It is involved in various cellular functions, such as cell structure and movement, cell communication and the functioning of the blood­brain barrier. It also plays a role in mitosis by adjusting the filament network present in the cell. Defects in GFAP are a cause of Alexander disease, a rare disorder of the central nervous system affecting mostly males. It is a progressive leukoencephalopathy resulting in mental and physical retardation, dementia, seizures and early death.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and RIA. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Frozen and paraffin sections. Requires protein digestion pretreatment of paraffin sections e.g. trypsin or pronase.
Optimal dilutions to be determined by the researcher.

Positive Control for Immunohistochemistry:
Brain

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.