GLUT3 (Solute Carrier Family 2, Facilitated Glucose Transporter Member 3, Glucose Transporter Type 3, Brain, GLUT-3, SLC2A3)

Antibodies / Antibodies-Glucose Transporters

-20°C

Supplied as a liquid in PBS, pH 7.5. No preservative added.

Recombinant protein corresponding to human GLUT3.
Epitope: Extracellular

Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes 7 proteins (Glut-1 to Glut-13, Mol. Wt. 40-80kD) and Sodium glucose co-transporter-1 (SGLT-1, 662aa; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics.
GLUT3 (human 496aa, mouse 493aa) is the main transporter in neurons, whereas Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: ~10ug/ml labels 10e6 cells. Perform the antibody binding in small volume of ~200ul.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.