Heat Shock Protein 60 (HSP60, GroEL, Chaperonin 60, cpn60)

Antibodies / Antibodies-Heat Shock Proteins

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Recombinant human HSP60 (1-573aa) purified from E. coli.

Heat shock protein 60 (HSP60) is a mitochondrial chaperonin that is typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. HSP60 is the ~60kDa mammalian equivalent to GroEL of E.coli. Process of HSP60 is regulated by the cochaperonin HSP10, a single heptameric ring of ~10kD subunits that forms a complex with HSP60. HSP10 coordinates the ATPase activity of the HSP60 subunits to allow the release of bound polypeptide in a manner productive for folding.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000-1:3, 000
Western Blot: 1:1000-1:3, 000
Optimal dilutions to be determined by the researcher.

Hybridoma:
Mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HSP60 protein.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.