Heat Shock Protein 70, Mitochondrial (HSPA9B, mtHSP70, GRP75, Mortalin 2)

Antibodies / Antibodies-Heat Shock Proteins

4°C Do Not Freeze

Supplied as a lyophilized powder from 20mM Tris, pH 7.4, 0.15M sodium chloride, 5% trehalose. Reconstitute with 100ul sterile PBS, 0.02% SODIUM AZIDE.

Recombinant corresponding to aa542-679 of human GRP75
expressed in E. coli (Accession # NP_004125).

The 70kD heat shock proteins (HSP70s) are a highly conserved family of stress response proteins. The HSP70 family of proteins contains both heat/stress inducible and constitutively expressed members known as heat shock As reportedcognate proteins. Glucose Regulated 75kD Protein (is a 679 amino acid heat shock cognate protein. Many HSPs function as molecular chaperones, facilitating the folding of other cellular proteins. GRP75 is a mitochondrial protein involved in protein translocation into the mitochondria. Proteins crossing the mitochondrial membrane require unfolding before entering translocation pores in the mitochondrial outer membrane. GRP75 together with other inner membrane proteins of the mitochondria mediate this process. GRP75 also plays a role in the control of cell cycle progression.

Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 0.25ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. DO FREEZE. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.