Heat Shock Protein 60 (HSP60, HSP-60, 60kD Heat Shock Protein Mitochondrial, 60kD Chaperonin, Chaperonin 60, Cpn60, GroEL, HuCHA60, HLD4, HSP65, HSPD1, Mitochondrial Matrix Protein P1, P60 Lymphocyte Protein, SPG13)

Antibodies / Antibodies-Heat Shock Proteins

-20°C

Supplied as a liquid in PBS, pH 7.4, 50% glycerol.

Synthetic peptide mapping at the middle region of human HSP60, identical to the related rat and mouse sequence.

Hsp60 is a member of a highly conserved family which includes molecular chaperones. Consistent with their functions as chaperones, Hsp60 and Hsp10 have been suggested to act as docking molecules with a passive role in the maturation of caspase processing, and are primarily known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria . It is now clear that a significant amount of HSP60 is also present in the cytosol of many cells and that it is induced by stress, inflammatory and immune responses, autoantibodies correlated with Alzheimer's, coronary artery diseases, MS, and diabetes.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml
Immunohistochemistry (frozen, paraffin): 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.