HGF, CT (Hepatocyte Growth Factor, Scatter Factor, SF, Hepatopoeitin A, HGF, HPTA) (Biotin)

Antibodies / Antibodies-Growth Factors, Cytokines

-20°C

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa521-554 in the C-terminal region of human HGF, UniProt Accession #NP_000592

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kD alpha-chain and 34-kD beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. Transcript Variant: This variant encodes the longest isoform. To date, experimental evidence for cleavage of the proprotein into two mature chains has been shown only for isoform 1.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other potential application, though not tested is Direct Flow Cytometry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Formalin-fixed, paraffin-embedded sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.