Hexokinase 2 (HXK2, HK2, Hexokinase Type II, HK II, Muscle Form Hexokinase)

Antibodies / Antibodies-Enzymes

-20°C

Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

Synthetic peptide corresponding to 17aa of mouse HKX2/HK2, N-terminal (KLH).

Hexokinase catalyzes the first step of several metabolic pathways by converting D-hexose to D-hexose-6-P. Hexokinase is an allosteric enzyme inhibited by its products glucose-6-phosphate. At least 4 related hexokinase isoforms (HXKI-III; HXK-IV also known as Glucokinase) have been cloned and characterized. Hexokinases (~100kD for HXK1-III; HXKIV lacks the N-terminal domains and is ~50kD) are outer mitochondrial membrane proteins. The N-terminus, containing the mitochondrial target sequence, and the C-terminal has high sequence homology among various isoforms. The catalytic activity is associated with the C-terminus and other regulatory functions are controlled by the N-terminus. HXKII is located on chromosome 2. HXKII is the predominant isozyme that is expressed in young red cell, the skeletal muscle, adipose tissue, and reticulocytes. It is regulated by insulin. Defects in HXKII are a cause of monogenic autosomal dominant non-insulin-dependent diabetes mellitus type II (MODY-II or NIDDM).

Applications:
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-1ug/ml
Western Blot: 1-10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.