His-Tag (6-Histidine) (AP)

Antibodies / Antibodies-Epitope Tag, His

4°C Do Not Freeze

Supplied as a liquid in 0.09% sodium azide. Labeled with Alkaline phosphatase (AP).

His-tag-fusion protein.

Recombinant DNA technology allows the addition of short pieces of well-defined tags, "peptides" or proteins at the amino or C-terminus of target genes, which can provide 'affinity handles' designed to bind specific matrices. Tags enable a selective identification and purification of the protein of interest. Poly-His (His x 6) containing proteins can be purified by immobilized metal ion affinity chromatography (IMAC), making use of its high affinity for transition metal ions. This purification system eliminates the harsh conditions required to elute protein from ligand affinity columns. Numerous recombinant fusion proteins have been engineered with histidine tags to allow detection, isolation and purification of fusion proteins. Anti-poly may be used to identify the expression of a poly-histidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a poly-histidine fusion protein expression vectors.

Applications:
Suitable for use in ELISA, Dot Blot, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:10, 000-1:50, 000
Immunohistochemistry: 1:200-1:2000
Western Blot: 1:1000-1:10, 000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.