His-Tag (6-Histidine) (HRP)

Antibodies / Antibodies-Epitope Tag, His

-20°C

Supplied as a liquid, 0.05% merthiolate. Labeled with Horseradish peroxidase (HRP).

His-tag fusion protein

Recombinant DNA technology allows the addition of short pieces of well-defined tags, "peptides" or proteins at the amino or C-terminus of target genes, which can provide 'affinity handles' designed to bind specific matrices. Therefore, tags enables a selective identification and purification of the protein of interest. Poly-His (His x 6) containing proteins can be purified by immobilized metal ion affinity chromatography (IMAC), making use of its high affinity for transition metal ions. This purification system eliminates the harsh conditions required to elute protein from ligand affinity columns. Numerous recombinant fusion proteins have been engineered with histidine tags to allow detection, isolation and purification of fusion proteins. Anti-poly may be used to identify the expression of a poly-histidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a poly-histidine fusion protein expression vectors.

Applications:
Suitable for use in Dot Blot, Western Blot, ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:5000 using chemiluminescence technique.
ELISA: 1:10, 000-1:50, 000
Immunohistochemistry: 1:200-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.