Histone H3, trimethyl (Lys9)

Antibodies / Antibodies-Histones

-20°C

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.

A synthetic peptide containing the 2X-branched sequence …AR[me3K]ST… in which me3K corresponds to trimethyl-lysine at residue 9 of human histone H3 (KLH).

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the “beads on a string” structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Applications:
Suitable for use in Western Blot, Dot Blot, Peptide Inhibition Assay and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500 detected trimethyl Histone H3 in acid extracted proteins from HeLa cells.
Peptide Inhibition: Specificity of this lot confirmed by the ability of 10uM of the immunizing peptide to completely abolish detection of histone H3 in Western Blot of HeLa acid extracts. No inhibition of detection was observed by preabsorption of the antibody with 10uM histone H3 peptide containing mono- or dimethyl-lysine 9, or mono-, di- or trimethyl-lysine 27 modifications.
Optimal dilutions to be determined by the researcher.

Recommended secondary antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.