Histone H4, trimethyl (Lys20)

Antibodies / Antibodies-Histones

-20°C

Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H4 trimethylated at Lys20 (NP_001029249).

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H4 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Applications:
Suitable for use in Western Blot, Dot Blot, Immunoprecipitation and Chromatin Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 2ug/ml detected trimethyl-Histone H4 (Lys20) in10ug of BALB3T3/A31 acid extract and RAT2 acid extract.
Immunoprecipitation: Has been reported to immunoprecipitate chromatin containing trimethylhistone H4 (Lys20).
Chromatin Immunoprecipitation: A representative lot immunoprecipitated Histone H4 using chromatin from HeLa cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.