MAP1LC3B (LC3,Autophagy Related Protein LC3B,Autophagy Related Ubiquitin-like Modifier LC3B,MAP1 Light Chain 3-like Protein 2,Microtubule Associated Proteins 1A/1B Light Chain 3B,MAP1A/1B Light Chain 3B,MAP1A/MAP1B LC3B,

Antibodies / Antibodies-Microtubule-associated Proteins

-20°C

Supplied as a liquid in Tris-glycine, 150mM sodium chloride, pH 7.2.

A synthetic peptide made to an N-terminal portion of the human LC3 protein sequence (between residues 1-100).

LC3, a mammalian homologue of Apg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. However, LC3 is now thought to also be involved in autophagy. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. LC3-II is the first mammalian protein identified that specifically associates with the autophagosome membranes.

Cellular Localization: LC3-I is cytoplasmic. LC3-II binds to the autophagic membranes.

Positive Control: Human/mouse brain lysate, ES, HeLa, HEK293, MCF7 cells, human lymphoblasts, and embryoid bodies

Applications:
Suitable for use in Western Blot and Immunohistochemistry (paraffin-embedded sections). Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.