LRRK2 (Leucine-rich Repeat Kinase 2, RIP7, PARK8, Dardarin)

Antibodies / Antibodies-Protein Kinases

-20°C

Supplied as a liquid.

A C-terminal synthetic peptide made to the human LRRK2 protein sequence (between residues 2500-2527).

This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8.

Applications:
Suitable for use in ELISA, Immunohistochemistry (Paraffin), Immunoflourescence and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:100
Immunofluorescence: 1:1000 (Mouse CAD cells transfected with human wild-type LRRK-2,
immunoprecipitation: 7ul/15 cm plate of SH-SY5Y cells grown to 80% conflue,
Western Blot: 1:1(the theoretical molecular weight of LRRK2 is ~286kD. Other bands have been seen with some lysates, but these bands have been blocked by the control peptide, L4502-21E, suggesting that these bands are degredation products)
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.