LRRK2 (Leucine-rich Repeat Kinase 2, RIP7, PARK8, Dardarin)

Antibodies / Antibodies-Protein Kinases

-20°C

Supplied as a liquid in Tris-citrate-phosphate, pH 7-8, 0.09% sodium azide.

A synthetic peptide made to an internal region of the mouse LRRK2 protein sequence (between residues 900-1000). [Swiss-Prot# Q5S006]

This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 2 ug/ml, A band is seen at ~284kD. There is a band at ~171 and 117kD, as well as several lower molecular weight, non-specific bands.
Immunohistochemistry: 2.5-5.0ug/ml, (Paraffin): 2.5-5.0ug/ml
Optimal dilution determined by the researcher.

Positive Controls: Mouse brain lysate

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.