MAP Kinase p44/42,phosphorylated (Thr202/Tyr204) (MAPK3/MAPK1,Mitogen-activated Protein Kinase 1,MAP Kinase 1,MAPK 1,ERT1,Extracellular Signal-regulated Kinase 2,ERK-2,MAP Kinase Isoform p42,p42-MAPK,Mitogen-activated Pr

Antibodies / Antibodies-Protein Kinases

-20°C

Supplied as a lyophilized powder in PBS, PEG, sucrose, 0.09% sodium azide. Reconstitute with 1ml ddH2O.

KLH-conjugated phosphopeptide containing the pTEpY motif of Erk1 and Erk2.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.

Recommended Dilution:
Western Blot: 0.1-1ug/ml detects pMAPK in RIPA lysates from EGF-stimulated human A431 carcinoma cells. Non-stimulated A431 and EGF-stimulated A431 cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-pMAPK (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: Use 1-10ug per immunoprecipitation.
Optimal dilutions to be determined by the researcher.

Positive Antigen Control:
EGF-stimulated A431 lysate. Use 20ug per lane for minigels. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.