MeCP2 (Methyl CpG Binding Protein 2 (Rett Syndrome), AUTSX3, MRX16, MRX79, PPMX, RTS, RTT) (MaxLight 550)

Pab

MaxLight 550

4°C Do Not Freeze

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.

KLH-conjugated, synthetic peptide corresponding to aa465-478 of mouse MeCP2 (C-PRPNREEPVDSRTP) with an N-terminal cysteine added for conjugation purposes

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150, 000.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Immunoblot Analysis: 0.5-2ug/ml of this lot detected MeCP2 in mouse heart nuclear extracts and a previous lot detected MeCP2 in mouse brain nuclear extracts and L6 nuclear extracts.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.