MCAM (Melanoma Cell Adhesion Molecule) (FITC)

Mab

IgG2b, k

-20°C

Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).

Pooled Ed15 and Ed16 thymocytes from H.B15, H.B19 and H.B21 chicken embryos

Chicken MCAM, a member of the immunoglobulin superfamily of cell surface receptors, consists of the V-V-C2-C2-C2 Ig domains. It is a single chain glycoprotein with three mRNA splice variants. One has a short cytoplasmic tail, another has a long tail, and the third seems to lack transmembrane and cytoplasmic regions. It is highly homologous to gicerin, a molecule involved in neurite outgrowth and Wilm’s kidney tumor progression in the chicken, and to the human melanoma progression molecule MUC18. Chicken MCAM is expressed by c-kit+ hematopoietic progenitor cells in embryonic bone marrow, thymocytes, and capillary endothelial cells. MCAM-expressing cells sorted from bone marrow are enriched in progenitors capable of differentiating into T cells when transferred into the appropriate thymic microenvironment. MCAM has been proposed to be involved in cellular adhesion and homing processes.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: ≤1ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.