MCP3 (Macrophage/Monocyte Chemotactic Protein 3)

Antibodies / Antibodies-Growth Factors, Cytokines

-20°C

Supplied as a lyophilized powder from PBS. Reconstitute with 500ul of sterile ddH2O.

Recombinant human MCP-3.

Applications:
Suitable for use in ELISA, Western Blot and Neutralization. Other applications have not been tested.
 
Recommended Dilutions:
Neutralization: 1-3ug/m Will yield one-half maximal inhibition [ND50] of the biological activity of hMCP-3 (100ng/ml)
ELISA (Sandwich): 2.0-4.0ug/ml (using 100ul/well antibody) Detection limit of 0.5-1.0ug/well antigen.
Western Blot: 0.5-1.0ug/ml. Detection limit is 2.0-4.0ng/lane, under reducing and 0.25-0.5ng/lane under non-reducing conditions.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Produced in C3H/HeJ mice.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Country of Origin:
USA