Nucleoredoxin (NXN, FLJ12614, NRX, TRG-4, Red-1)

Antibodies / Antibodies-Neuroscience

-20°C

Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute the antibody in 100ul PBS, 0.02% sodium azide. The antibody concentration will be 1mg/ml.

Purified, E. coli-derived, recombinant human Nucleoredoxin (rhNRX; aa 131-435; Accession# Q6DKJ4).

Nucleoredoxin (NRX; also known as NXN and Red-1) is a 51-55kD member of the thioredoxin (TXR) family of proteins. It is widely expressed, and exists in both the nucleus and cytoplasm of cells where it regulates two pathways. In the nucleus, NRX may act as a transcription factor in the Wnt-pathway; a stable (reduced) NRX binds to DVL, thereby blocking downstream Wnt/β-catenin signaling. It also binds to PP2A subunits PP2A C and PP2A A, generating an active PP2A complex. Human NRX is 435 amino acids (aa) in length and contains a TXR domain (aa 167-321) that shows a WCPPC catalytic site, and a substrate recognition PDI-b’ region (aa 312-427). There are three potential isoform variants. Over amino acids 131-435, human NRX shares 99% aa identity with mouse NRX.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.